Analysis of proportion of nucleic acids and proteins in glandular stomach wall in chickens during experimental escherichiosis
DOI:
https://doi.org/10.28983/asj.y2020i4pp44-50Keywords:
spectral analysis method, Stains all, nucleonic acids, proteins, glandular stomach chickens, experimental escherichiosisAbstract
A method for spectral analysis of the ratios of organic substances in a cell using the "Stains all" metachromatic luminescent dye (in its own modification) has been developed. This method is used to establish the ratio of nucleic acids and proteins in the integumentary epithelium of the mucous membrane and in the interlobular connective tissue of the submucosa tissue of the glandular stomach of chickens. Using the developed biophysical method, we studied the ratios of these organic substances in the glandular stomach of healthy and infected with E. coli chickens. The dynamics of the ratios of nucleic acids and proteins detected in the wall of the glandular stomach of the control group chicks fits into the picture of a moderate and uniform increase in the values of these indicators, corresponding to an age increase of the chickens. In chickens affected by Escherichiosis, a curve that reflects the dynamics of the ratios includes a three-fold increase in their values. The results obtained using the developed method indicate the possibility of using it to detect early metabolic changes in the glandular stomach of chickens before the appearance of a characteristic pathomorphological and clinical picture. Thus, this method can provide invaluable assistance in developing a fundamentally new approach in creation of modern technologies for the diagnosis, prevention and treatment of widespread disease Escherichiosis.
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