Assessment of destructive processes of chromatin in granulose of porcine ovarian fol-licules containing growing or fully grown oocytes
DOI:
https://doi.org/10.28983/asj.v0i9.578Keywords:
porcine granulosa, oocyte, BCB-test, apoptosisAbstract
Significant differences in character of atretic processes in porcine granulosa were established as a result of comparative monitoring of the viability and degeneration of chromatin (apoptosis, pycnosis) in granulosa cells from follicles containing growing or fully grown oocytes using cytology analysis, cytofluorometry (Annexin-test) and TUNEL-test. BCB-test was used for the evaluation of the functional state of the oocytes (EgerszegiI. etal., 2010). Completion of the oocyte growth phase was accompanied by a significant increase in the proportion of granulosa cells from the follicles with the fully grown oocyte in the status of apoptosis (11% vs 7%, P <0.05), pyknosis (9% vs 5%, P <0.05), necrosis (5% vs 3%, P <0.05) and a decreasing in the level of viable cells (85% vs 88, P <0.05) compared to the above markers in granulosa cells from follicles with growing oocytes. The revealed features of destructive processes in granulosa cells from follicles containing growing or fully grown oocytes supplement the available information on the features of the interaction of the germ and somatic cells of the ovarian follicle in the formation of an egg that is capable of fertilization and further development of the embryo.
Downloads
References
2. Кузьмина Т.И., Станиславович Т.И., Молчанов А.В. Апоптоз соматических клеток фолликулов и функциональный статус ооцитов BOSTAURUS // Аграрный научный журнал.– 2018. – № 5. – С. 30–35.
3. Egerszegi I., Alm H., J. Ratky B. Meiotic progression, mitochondrial features and fertilization characteristics of porcine oocytes with different G6PDH activities //
ReprodFertil Dev., 2010, V. 22, P. 830–838.
4. Feng W., Pan Z. The effect of granulosa cells apoptosis on the cumulus expansion and the developmental competence of bovine oocytes // Advanced Materials Research, 2014, V. 997, Р. 251–254.
5. Flisikowska T., Kind A. &Schnieke A. The new pig on the block: modeling cancer in pigs // Transgenic Res., 2013, V. 22, Р. 673–680.
6. Grupen C.G. The evolution of porcine embryo in vitro production // Theriogenology, 2014, V. 81, Р. 24–37.
7. Jolly P.D., Smith P.R., Heath D.A. et al. Morphological evidence of apoptosis and the prevalence of apoptotic versus mitotic cells in the membrane granulosa of ovarian follicles during spontaneous and induced atresia in ewes // Biol. Reprod., 1997, V. 56, P. 837–846.
8. Meyer M., Hrabe De Angelis М.,Wurst W. &Kuhn R. Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases // Proc. Natl.
Acad. Sci., 2010, V. 107, Р. 15022–15026.
9. Niemann H., Petersen B. The production of multitransgenic pigs: update and perspectives for xenotransplantation // Transgenic Res., – 2016, V. 25(3), P. 361–374.
10. Sato E. Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals // Proc. Jpn. Acad., 2015, V. 91, P. 76–91.
11. Tarkowski A. An air-drying method for chromosomal preparation from mouse eggs // Cytogenetic, 1966, V. 1, P. 394–400.
