The effect of polymorphism of PON1 gene on paraoxonase-1 levels and biochemical parameters of the blood serum in Holstein cattle
DOI:
https://doi.org/10.28983/asj.y2022i8pp61-65Keywords:
PON1 gene, PCR-RFLP, paraoxonase-1, blood serum, cattleAbstract
The redox state status serves a leading function in metabolism, affecting the stable course of many physiological reactions. Operation of intracellular enzymatic antioxidants is affected by both dietary and genetic control. The influence of high concentrations of the paraoxanase-1 enzyme has a positive effect, maintaining the body's resistance to oxidative stress. The aim of the research was to study the polymorphism of the promoter region of the PON1 gene (-211 A/G) in association with the biochemical parameters of the blood serum of Holstein cattle. DNA genotyping 148 Holstein cows were performed by PCR-RFLP. As a result of identification, three genotypes were established: AA – 31.8% (47 animals), GA – 49.3% (73 animals) and GG – 18.9% (28 animals), and the observed frequency of alleles A and G was – 0.564 and 0.436. An analysis of the relationship between the PON1 gene polymorphism and the level of paraoxonase-1 indicates that, depending on the genotype, the activity of these enzyme changes, as a result of which there is a variation in the biochemical parameters of the blood serum of Holstein cattle.
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